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. 2012 Dec 26;7(12):e52716. doi: 10.1371/journal.pone.0052716

Figure 4. Determination of Sp1 binding to the USP22 gene promoter.

Figure 4

A. DNA was isolated from HFL1 cells in each group and immunoprecipitated with antibodies against Sp1, RNA polymerase II or nonspecific rat IgG. Input and immunoprecipitated DNAs were then PCR amplified using primer pairs covering the Sp1-binding site. B. HFL1 cells expressing p-210 or p-210/Sp1mut constructs were treated with 10 um of mithramycin A or vehicle. Luciferase activity was determined 24 h later (*, p<0.05 vs. vehicle treatment).