Figure 5. The SlitBest1b current depends on the Ca²⁺ concentration and is anionic. A.

) Dose-response recordings were conducted in a low-Cl⁻ external solution. The curve fitted to the Hill equation has an EC₅₀ of 1.2 µM and a Hill coefficient of 1.0. Means ± SEM. Representative currents recorded during voltage step protocols and corresponding I-V relationships from SlitBest1b transfected cells dialyzed with 100 µM intracellular Ca²⁺, B) in symmetrical Cl⁻ concentrations (E _Cl = 0 mV), and C) in low-Cl⁻ bath solution (E _Cl =104 mV). Dotted horizontal lines are zero current levels.