Table 1. List of the primers used for single-cell reverse transcription polymerase chain reaction (RT-PCR).
Sense primer | Antisense primer | ||
SlBest1a-SC.F | 5′-CCGTGGCGTGAAACTGAAGAAA (PWRETEE) | SlBest1-SC.R1 | 5′-ACTCTTGGTGAGATCATCGTGAGC (LTMISPR) |
SlBest1b-SC.F | 5′-CGAGGTAGCAACTTGTCGTGGTTT (EVATCRG) | SlBest1-SC.R2 | 5′-GTAAGGCAGAGACACACGTAGCGG (RYVCLCL) |
SlBest2-SC.F | 5′-CGGCAGCAGTTTCGGATGTTTCTG (GSSFGCF) | SlBest2-SC.R1 | 5′-CGACCCGTCTCGTCTTTACTAACC (VSKDETG) |
SlBest2-SC.R2 | 5′-ATGTTGCCACGTTGGAAACCTTCG (RRFPTWQH) | ||
SlOrco-SC.F | 5′-CTTTATCTGCGGCATGACTGTCCT | SlOrco-SC.R1 | 5′-ACTGCACCAAGTACCGAAGCAA |
SlOrco-SC.R2 | 5′-TCGGAGTCAAGCCATTAGGGTTGT | ||
RpL8-SC.F | 5′-GTGATTCGTGCTCAGCGTAAAGGT | RpL8-SC.R1 | 5′-TGAGGATGCTCAACGGGGTTCATA |
RpL8-SC.R2 | 5′-CCAATGACAGTGGCGAAGTTTCCT |
Corresponding amino acid sequences are indicated between brackets.