Table 2. Compositions of pipette solutions for dose-response experiments.
| Free-Ca2+ (M) | NMDG-Cl (mM) | CaCl2 (mM) | EGTA (mM) | HEDTA (mM) | NTA (mM) |
| 2×10−8 | 122.8 | 1.1 | 11 | – | – |
| 1×10−7 | 117.8 | 3.6 | 10 | – | – |
| 1×10−6 | 122.4 | 1.3 | – | 10 | – |
| 1×10−5 | 113 | 6 | – | 10 | – |
| 1×10−4 | 121.4 | 1.8 | – | – | 5 |
| 1×10−3 | 123 | 1 | – | – | – |
All solutions contained 10 mM HEPES. The pH was adjusted to 7.2 with NMDGOH and osmotic pressure was 290 mosmol/L. Free Ca2+ concentrations were calculated with WebmaxC Standard (http://www.stanford.edu/~cpatton/webmaxc/webmaxcS.htm).