Table 4. Primers used in this study.

No.	Primers	Sequence
1	CLC–EcoRI-A	GGG TGA ATT CCG AAG GAT TGA GGC TGA GCA AG
2	CLC–XhoI-S	CCT TCT CGA GTG TTT CTC CAG TTC ACC ACC CTG
3	CLC-mi1 -HindIII	CCC GAA GCT TTC CTG AAC TTG ATC ACC ATT GAT CAT CCT TGT ATT CCT TCG TCC
4	CLC-mi1-mut-HindIII	CCC GAA GCT TTC CTG AAT CCA GCT GTT ATT GAT CAT CCT TGT ATT CCT TCG TCC
5	CLC-mi2 -HindIII	CCG CAA GCT TTT ATC GAC ACC TTC ATC GTA ATC ATC CTT GTA TTC CTT CGT CC
6	CLC-mi2-mut-HindIII	CCG CAA GCT TTT ATC GGT GTT CCT GCT GTA ATC ATC CTT GTA TTC CTT CGT CC
7	CLC-HindIII-S	CCC CGA AGC TTA AAT TTT GTT AGA CTG ACC AG
8	URA5-XhoI-S	GGG ACT CGA GGA TCT TGG GGA TGG TAT TGA AGA CG
9	URA5-XbaI-A	CTC GTC TAG AGA TCC CAG TAC TAC CCG CTC TT
10	URA5-mi1-BamHI	GCC CGG ATC CTC CTG AAC TTG ATC ACC ATT GAT TAA GAC CTC TGA ACA CCG TAC
11	URA5-mi1-mut-BamHI	GCC CGG ATC CTC CTG AAT CCA GCT GTT ATT GAT TAA GAC CTC TGA ACA CCG TAC
12	URA5-mi2-BamHI	GCC CGG ATC CTT ATC GAC ACC TTC ATC GTA ATT AAG ACC TCT GAA CAC CGT AC
13	URA5-mi2-mut-BamHI	GCC CGG ATC CTT ATC GGT GTT CCT GCT GTA ATT AAG ACC TCT GAA CAC CGT AC
14	URA5-BamHI-S	TGG GGA TCC GGG TTT TCT TCT TAA ATG CAC GGG
15	qURA5	TAC AGG AGG TTG AGG AAG A(up)
		TTA AGA CCT CTG AAC ACC G(down)
16	qCLC1	AAT GAC TGT GAA TAC GGG CG(up)
		CTT GGT CGG ACA CGA GAA TG(down)
17	qACT	ATG GTA TTG CCG ACC GTA TGC(up)
		TTT CGG TGG ACG ATT GAG GG(down)