FIG. 2.

Effect of zinc salts on RSV yield when present during adsorption, penetration, and egress. Virus was combined with various concentrations of zinc salt or control salts in 50 mM MOPS-buffered Opti-MEM I medium, pH 7.2, in a total volume of 250 μl, and the mixture was incubated at 37°C for 2 h. Control wells without zinc (0 mM) were treated similarly. At the end of the incubation period, the zinc-virus mixtures were inoculated onto HEp-2 cell monolayer cultures. After 96 h supernatants were collected and the viral titer was determined by standard 4-day plaque assay on HEp-2 cell monolayer cultures. Virus titer values indicate the mean values and SEM of three independent experiments.