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. 2012 Dec 26;7(12):e52430. doi: 10.1371/journal.pone.0052430

Figure 4. The endocytic pathways of Gal-3 in HUVEC.

Figure 4

A: The cells were incubated with DTAF-Gal-3 and Cy3-transferrin (only at 30 min) at 37°C for 10, 30, or 120 min and were then processed for IF analysis with antibodies against EEA1, Lamp-1 and Golgin-97. B: The cells were incubated with DTAF-Gal-3 at 4°C for 60 min. After washing, the cells were either fixed immediately (0 min) or transferred to 37°C for 5 min or 10 min and then fixed. C: The cells were incubated with DTAF-Gal-3 and Cy3-transferrin at 20°C for 60 min. The images were obtained with a confocal microscope (A and B) or a fluorescence microscope (C). Scale bar, 10 µm.