Figure 6. Gal-3 did not associate with HUVECs in the absence of the CRD.

A: The HUVECs were incubated with 15 µg/ml DTAF-GST-Gal-3 or DTAF-GST-G1-108 at 37°C for 120 min and examined with a confocal microscope. Scale bar, 10 µm. B: The HUVECs were incubated with 15 µg/ml GST-Gal-3 or 30 µg/ml GST-G1-108 at 37°C for 30 min. The amount of proteins added to the medium is shown in the top panel (Medium). The cells were extensively washed and analyzed by western blotting with antibodies to Gal-3 and actin.