Figure 4. Transfection with NTS-polyplex requires NTSR1 activation.

The NTSR1 antagonist SR48692 was used at a concentration of 100 nM. In panel A, the micrographs illustrate that the NTSR1-antagonist blocked pDsRed2 transfection. The neurons were processed for immunocytochemistry against TH (green) and DsRed (red). The transfections were performed at 1∶30 molar ratio in 8-day-old neurons in the presence of complete culture medium with serum. Panel B shows the efficiency of transfection for each condition. Negative controls were prepared using NTS-carrier without plasmid. The cell counting was performed from 30 fields per coverslip (n = 3). The images were acquired using a 20× objective. *P<0.05. Scale bar = 50 µm.