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. 2012 Dec 26;7(12):e51341. doi: 10.1371/journal.pone.0051341

Figure 6. Decreased cell viability after VGLUT2-Venus transfection using the NTS-polyplex.

Figure 6

Transfection of pVGLUT2-Venus using the NTS-polyplex caused substantial damage to both neurons and astrocytes at the three molar ratios tested. Panel A shows phase contrast micrographs illustrating the appearance of DA neurons and astrocytes after transfection using three molar ratios. In B, the graph depicts the average number of TH-positive DA neurons relative to non-transfected controls (%). The neurons were processed for TH immunocytochemistry (green). Negative controls were exposed to NTS-carrier without plasmid. The cell counting was performed from 30 fields per coverslip (n = 3). The images were acquired using a 20× objective. *P<0.05. Scale bar = 50 µm.