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. 2012 Dec 26;7(12):e51189. doi: 10.1371/journal.pone.0051189

Figure 1. Dual inhibition of IR/IGF-1R and SFK decreases tumor growth in vitro more effectively than single pathway inhibition does.

Figure 1

(A) PC-3 or LNCaP cells were cultured for 96 hours with and without dasatinib (DSA; 100 nM) and BMS-754807 (BMS-807; 2 µM for PC-3 and 0.5 µM for LNCaP cells), alone and in combination, and cell numbers were determined as described in the methods. (B) PC-3 and LNCaP cells were incubated for 24 hours with and without DSA (100 nM) and BMS-754807 (5 µM for PC-3 and 1 µM for LNCaP cells), alone and in combination, and cell cycle staining after 24 hours was performed using propidium iodide. (C) PC-3 cells were incubated for 48 hours with and without DSA (100 nM) and BMS-754807 (2 µM), alone and in combination, and the percentage of apoptotic cells was determined by flow cytometric analysis of annexin-V staining. (D)Left: cell cycle staining with propidium iodide in PC-3–shIGF-1R cells. Right: PC-3–shIGF-1R and control cells were incubated for 48 hours with and without DSA (100 nM), and the percentage of apoptotic cells was determined by flow cytometric analysis of annexin-V staining. All experiments were performed in triplicate. *P<0.05; N.S. not significant.