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. 2012 Dec 26;7(12):e52398. doi: 10.1371/journal.pone.0052398

Figure 4. Histopathological analysis of organs from melphalan, vehicle control treated and untreated mice confirms in vivo BLI data.

Figure 4

(A) Representative H&E stainings from organs harvested at day +33 after tumor inoculation from the different treatment groups. The hematopoietic compartments BM and spleen displayed clear infiltration of MOPC-315.BM luc+ cells, while MOPC-315.BM luc+ cells in lung and liver resided within the blood vessels as indicated by arrows. Scale bar is 50 µm for all shown sections (original magnification 200X/0.70 NA) and 20 µm for inserts (original magnification 400X/0.85 NA). (B) Determination of the percentage of MOPC-315.BM luc+ cells within BM and spleen and (C) number of cells per high power field (HPF) in lung and liver significantly correlates with the melphalan treatment and thereby confirms in vivo and ex vivo imaging data.