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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Vascul Pharmacol. 2012 Aug 23;58(1-2):87–97. doi: 10.1016/j.vph.2012.08.003

Figure 5.

Figure 5

PDBu-stimulated cytoskeletal remodeling of: (A) untreated A7r5 cells placed in conditioned media collected from culture of nicotine-treated A7r5 cells, and (B) extensively washed nicotine-treated A7r5 cells in fresh media. In panel A, donor A7r5 cells were treated with 2 μM nicotine for 6 hr to produce the conditioned media, which was collected to treat recipient A7r5 cells for 1 hr, followed by 1 μM PDBu stimulation for 1 hr. In panel B, A7r5 cells were treated with 2 μM nicotine for 6 hr, washed in fresh media 5 times, incubated in fresh media for 1 hr, and then stimulated with 1 μM PDBu for 1 hr. F-actin was labeled in red. Vinculin was labeled in green.