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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2012 Aug 25;1834(1):65–70. doi: 10.1016/j.bbapap.2012.08.017

Figure 1.

Figure 1

An OC-positive species is observed in freshly-purified Aβ(1-42) monomer fractions. Panel A. Aβ(1-42) was reconstituted in NaOH followed by dilution in F-12 medium. The solution was then centrifuged and the supernatant separated on a Superdex 75 column in F-12 medium. The elution shown is a representative experiment of numerous Aβ SEC purifications. Panel B. Aβ monomer fractions from separate SEC purifications were placed on ice immediately after elution from a Superdex 75 column and examined by dot blot analysis within 30 min. The concentration for monomer fractions of Aβ(1-42) and Aβ(1-42) L34P eluted in 50 mM Tris-HCl pH 8.0 was 20 μM and 40 μM respectively, while Aβ(1-40) monomer eluted in PBS was 78 μM.