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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2012 Aug 25;1834(1):65–70. doi: 10.1016/j.bbapap.2012.08.017

Figure 3.

Figure 3

OC-positive Aβ(1-40) species do not elute in the monomer fractions. Panel A. Aβ(1-40) (1.4 mg) was reconstituted in 50 mM NaOH followed by dilution in 50 mM Tris-HCl pH 8.0 to a concentration of 234 μM. The solution was incubated for 24 hours at 25° C, centrifuged at 18,000g for 10 minutes, and the supernatant was eluted on a Superdex 75 column. A continuous 280 nm absorbance elution trace (solid line) and collected 0.5 mL fractions (open circles) are shown. Panel B. Dot blot analysis was performed with Ab9 and OC antibodies for samples taken from the 24 hour supernatant pre-load (40 μM), protofibrils (fraction 13/14 pool) (30 μM) and monomer (fraction 21) (40 μM).