TABLE 1.
Validation data for standard curves for DHA spiked in plasma used in the bioassay
| Actual concn (ng/ml) | Intra-assay (n = 5)
|
Interassay (n = 6)
|
||||
|---|---|---|---|---|---|---|
| Concn found (mean ± SD) | % CVa | % Error | Concn found (mean ± SD) | % CV | % Error | |
| 1.25 | 1.50 ± 0.04 | 2.46 | 20.19 | NDb | ||
| 2.50 | 2.19 ± 0.24 | 10.86 | (12.56)c | ND | ||
| 5.00 | 5.12 ± 0.32 | 6.26 | 2.50 | 4.89 ± 0.81 | 16.47 | (2.18) |
| 12.50 | 12.46 ± 0.87 | 6.96 | (0.35) | 12.20 ± 1.10 | 8.29 | (2.44) |
| 25.00 | 25.13 ± 2.20 | 8.76 | 0.52 | 24.08 ± 2.03 | 8.45 | (3.69) |
| 50.00 | 50.23 ± 5.19 | 10.34 | 0.46 | 53.02 ± 3.70 | 6.97 | 6.04 |
| 100.00 | 111.30 ± 4.36 | 3.92 | 11.30 | ND | ||
a
CV is a measure of relative variation which expresses the SD as a percentage of the mean.
b
ND, not determined.
c
Negative values are in parentheses.