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. 2013 Jan;23(1):46–59. doi: 10.1101/gr.138842.112

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© 2013, Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported License), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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Figure 8. — Subcellular localization of N- and C-terminal FLAG-tagged LRRC37A. (A) HeLa cells transiently transfected with N- and C-terminal FLAG-tagged LRRC37A constructs and probed with anti-FLAG antibody. LRRC37A tagged at the N-terminus does not show strong localization, whereas LRRC37A tagged at the C-terminus shows a plasma membrane localization (magnification, 63X). (B) Western blot of lysates and conditioned media of HeLa cells transfected with FLAG-tagged LRRC37A. Lysates and conditioned media of HeLa cells transiently transfected with N-terminal (N) or C-terminal (C) FLAG-tagged LRRC37A, probed with anti-FLAG (lanes 1–3) and anti-LRRC37 (lanes 4–6) antibodies. Nontransfected HeLa cells represent the control. (C) Conditioned media were concentrated, and a comparable amount of lysates and media was loaded. Samples were probed with anti-LRRC37 antibody.