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H&E staining (before the fatigue test). (A) Glutaraldehyde fixation only; (B) glutaraldehyde fixation+decellularization; (C) glutaraldehyde fixation+decellularization+α-galactosidase. B and C had no visible cells. The interstitial matrix of B and C was less dense than that of A (H&E, ×400).
