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H&E staining (after the fatigue test). (A) Glutaraldehyde fixation only; (B) glutaraldehyde fixation+decellularization; (C) glutaraldehyde fixation+decellularization+α-galactosidase. A, B, and C had less dense interstitial matrix than pre fatigue test ones after the fatigue test than before. B and C showed relative derangement (H&E, ×400).
