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3.3'-diaminobenidine (DAB) staining. (A, D) Glutaraldehyde fixation only; (B, E) glutaraldehyde fixation+decellularization; (C, F) glutaraldehyde fixation+decellularization+α-galactosidase. (A-C) Background image; (D-F) α-gal signal. D, E, and F were α-gal signal images. F had no α-gal signal (×400).
