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Combinations of gene-specific primers for semiquantitative real-time PCR
| Chicken | Forward and Reverse Primers (5′-3′) |
|---|---|
| KCC1 | TCCCATGATGCCAGACTTGT |
| ACTCTCTCCAGACCCTCAGT | |
| KCC2 | GAGTCGGCCCCTGAGAAG |
| AAGTCCTTGATGCCCTCGG | |
| KCC2a | GAGAGCCGCCCGCAC |
| TCCAGGTCTGTGCTGTTGA | |
| KCC2b | AACCTGACGGACTGCGAG |
| GGGCTGGTGTCCATCTCC | |
| KCC4 | GTTCTTGTACCATCTCCGCC |
| GGGATCGCTGTTCCATCATT | |
| GAPDH | CTTTCCGTGTGCCAACCCCC |
| GCCCATCAGCAGCAGCCTTC |
