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. 2012 Oct 3;303(11):C1198–C1205. doi: 10.1152/ajpcell.00284.2012

Fig. 4.

Fig. 4.

Cab39 expression enhances constitutively active SPAK monomer. Ouabain-resistant unidirectional (86Rb) K+ fluxes were measured in groups of Xenopus oocytes injected with NKCC1 cRNA alone or in combination with concatamers of a constitutively active SPAK monomer (shaded blue) linked to either a wild-type or a mutant SPAK(T243A) monomer (shaded orange) and Cab39. Residues T243 and S383 are labeled in the semicircles and residue T247 is labeled in the center spheres. A zig-zag line represents the 9-glutamine residue linker connecting the two monomers. Note that last two bars represent NKCC1 activity in the presence of constitutively active SPAK without and with Cab39. Fluxes are expressed in nmol K+·oocyte−1·h−1. Bars represent means ± SE (n = 20–25 oocytes). Lines indicate significance between concatamer groups determined with one-way ANOVA (P < 0.001). *Additional significant differences within concatamer groups with Cab39 (P < 0.001).