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. 2013 Jan 1;24(1):1–13. doi: 10.1091/mbc.E12-03-0217

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© 2013 Chuang et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Y14/Magoh specifically associates with mRNA-decapping factors. HEK293 cells were transfected with the expression vector encoding a FLAG-tagged EJC core alone or together with a vector for expressing HA-Upf3b, HA-TAP, or myc-Dcp1a. Immunoprecipitation was performed in the presence of RNase A. Cell lysates were subjected to immunoprecipitation (IP) using anti-FLAG. Immunoblotting was subsequently performed using antibodies against HA or c-myc epitope, or Dcp2, Edc4, or DDX3. Bottom, one set of representative data for anti-FLAG immunoblotting.