FIGURE 1:

Activities of N-Srv2 and C-Srv2 in nucleotide exchange on G-actin and Cof1-mediated disassembly of F-actin. (A) Coomassie Blue–stained gel and schematic of Srv2 polypeptides used for biochemical analysis. Black dots indicate locations of point mutations in the srv2 alleles used in this study (90, 91, 98, 109; Chaudhry et al., 2010; Mattila et al., 2004; Quintero-Monzon et al., 2009). (B) Effects of 100 nM FL-Srv2, N-Srv2, and C-Srv2 on rate of ε-ATP exchange on 2 μM ADP-actin monomers in the presence of 5 μM cofilin. (C–E) F-actin depolymerization assays induced by vitamin D–binding protein. Final concentrations: 2 μM F-actin, 100 nM CapZ, 50 nM Cof1 or Cof1-9, and 100 nM Srv2 polypeptides.