TABLE 3.
In vitro peptidoglycan biosynthesis with membrane preparations from B. megateriuma
Inhibitorb | Peptidoglycan synthesis (%) by B. megateriumc |
---|---|
None (control) | 100 |
Tunicamycin | 0 |
Moenomycin | 17 |
Arylalkylidene rhodanine derivative 1 | 61 |
Arylalkylidene iminothiazolidin-4-ones | |
11 | 57 |
13 | 64 |
16 | 63 |
Peptidoglycan synthesis started with UDP-MurNAc-pentapeptide and UDP-[14C]-GlcNAc as substrates.
Each inhibitor was used at a concentration of 100 μg/ml.
The total incorporation of radioactive S in peptidoglycan in the control experiment without inhibitor was 1.5 pmol (12%).