TABLE 3.
In vitro peptidoglycan biosynthesis with membrane preparations from B. megateriuma
| Inhibitorb | Peptidoglycan synthesis (%) by B. megateriumc |
|---|---|
| None (control) | 100 |
| Tunicamycin | 0 |
| Moenomycin | 17 |
| Arylalkylidene rhodanine derivative 1 | 61 |
| Arylalkylidene iminothiazolidin-4-ones | |
| 11 | 57 |
| 13 | 64 |
| 16 | 63 |
Peptidoglycan synthesis started with UDP-MurNAc-pentapeptide and UDP-[14C]-GlcNAc as substrates.
Each inhibitor was used at a concentration of 100 μg/ml.
The total incorporation of radioactive S in peptidoglycan in the control experiment without inhibitor was 1.5 pmol (12%).