The morphological changes of RPE cells were observed by phase contrast microscope and Rapamycin(90ng/mL), LY294002(30µmol/L) A: Untreated Human RPE D407 cells; B: Compared with untreated cells, Rapamycin(90ng/mL) treatment (24 hours), the apparent change in cells became smaller length, and appeared floating cells; C: Compared with untreated cells, LY294002 treatment (24 hours), the apparent change in cell morphology, become smaller length, and appeared floating cells.