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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: J Immunol. 2012 Dec 5;190(1):159–167. doi: 10.4049/jimmunol.1201657

Figure 6.

Figure 6

Effect of SMRT knockdown on cytosolic and nuclear signaling. A: CD4 T cells were transfected with siSMRT and siNT Smartpool siRNA and then stimulated with and without (medium) anti-CD3/CD28 antibodies for 10 min and then western blotted for p-p38 and pERK1/2. The p-p38 membrane was reprobed for p38, and the pERK1/2 membrane was reprobed for beta-actin. (N=4). B: CD4 T cells were transfected with siSMRT and siNT siRNA and then stimulated with anti-CD3/CD28 antibodies for 8 and 48 hr. Nuclear extract was isolated and western blotted for p65 NFkB and JunD. The membrane was reprobed for HDAC3 (N=4).