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. Author manuscript; available in PMC: 2012 Dec 27.
Published in final edited form as: J Cell Physiol. 2008 Dec;217(3):752–758. doi: 10.1002/jcp.21550

Figure 2. PAK1 Activation is downstream of Ras.

Figure 2

Day 4 mature osteoclasts were pre-treated with a farnesyl transferase inhibitor to block Ras activation or vehicle control for seven hours. Cells were serum starved and treated with 25 ng/ml M-CSF as indicated in the presence of inhibitor or vehicle control. Western blotting for phospho-PAK1Thr423, total PAK1/3, phospho-MEK1/2Ser217/221, total MEK1/2, phospho-ERKThr202/Tyr204, total ERK1/2 and tubulin was then performed.