Table 2. Endogenous Peptides, Corresponding Internal Standards, and Selected Transitions For Quantification of Heat Shock Proteins in RPMI-8226 Multiple Myeloma Cells.
Underlined amino acid residues are stable-isotope labeled with 13C and 15N in the corresponding synthetic standards. Student’s t-tests were used for calculation of p values. Roman numerals indicate the corresponding ion signals in Figure 2, panels B–J.
| Protein (UniProt Identifier) | Endogenous Peptide | Transitions | Pre-Treatment (amol/cell) | Post-Treatment (amol/cell) | Ratio | p value |
|---|---|---|---|---|---|---|
| HS90A | ALLFVPR (IV) | y3 - y6 | 11.2 | 20.9 | 1.87 | 2.9E-5 |
| HS90B | ALLFIPR (V) | y3 - y6 | 7.90 | 14.5 | 1.84 | 4.4E-4 |
| H90B2 | HSQFLGYPITLYLEK (IV) | y3 -y12 | 16.1 | 25.3 | 1.57 | 3.2E-4 |
| CDC37 | LQAEAQQLR (I) | y4 – y7 | 1.08 | 0.91 | 1.07 | 0.038 |
| HSPB3 | ADLINNLR (I) | y3 - y7 | 0.11 | 0.14 | 1.25 | 0.026 |
| HSP71 | NQVALNPQNTVFDAK (I) | y3, y4, y9 - y12 | 0.013 | 0.051 | 3.95 | 1.2E-5 |
| HSP72 | EIAEAYLGGK (I) | y3 - y8 | 0.27 | 0.39 | 1.21 | 0.11 |
| HSP7C | GTLDPVEK (I) | y3 - y7 | 7.72 | 14.9 | 1.93 | 2.2E-6 |
| HSP74 | AFSDPFVEAEK (III) | y4, y6 - y9 | 0.73 | 0.89 | 1.23 | 1.3E-3 |
| GRP78 | VEIIANDQGNR (I) | y4 - y9 | 48.8 | 110 | 2.25 | 2.7E-4 |