TABLE 2.

Next nucleotide inhibition of ATP-mediated TFV excision by wild-type and mutant HIV-1 RT enzymes

HIV-1 RT	IC50 (μM) for next nucleotidea	% TFV removed in 5 μM next nucleotideb
Wild type	1.2 ± 0.2 (1.0)c	5 (1.0)
FS-SSS	14.1 ± 1.9d (11.8)	59 (12)
FS	4.6 ± 1.3e (3.8)	17 (3.4)

^aIC₅₀s were determined at 30 min and are the means ± standard deviations of three experiments.

^bPercentage of tenofovir removed at 30 min in 5 μM dATP.

^cValues in parentheses are the fold change from the value for the wild type.

^dP < 0.01 compared to the value for the wild type by two-tailed Student's t test.

^eP < 0.05 compared to the value for the wild type by two-tailed Student's t test.