Table 2.
Number of selected junctions and percentage of selected junctions that were validated by at least two 454 reads, as a function of the RNA-seq read count per junction
| H1ES |
HUVEC |
||||
|---|---|---|---|---|---|
| Read count per junction from two replicates | Number of tested junctions | Proportion of junctions validated by at least two 454 reads (%) | Read count per junction from two replicates | Number of tested junctions | Proportion of junctions validated by at least two 454 reads (%) |
| 2 | 192 | 72.4 | 2 | 192 | 74.0 |
| 3 | 192 | 77.6 | 3 | 192 | 75.0 |
| 4 | 96 | 74.0 | 4 | 96 | 76.0 |
| 5 | 96 | 82.3 | 5–6 | 96 | 84.4 |
| 6–7 | 96 | 79.2 | 7–8 | 96 | 84.4 |
| 8–11 | 96 | 81.3 | 9–12 | 96 | 86.5 |
| 12–24 | 96 | 87.5 | 13–23 | 96 | 94.8 |
| ≥25 | 96 | 88.5 | ≥24 | 96 | 90.6 |