Figure 4.

Attachment of eGFP⁺ HCT116-19 cells onto PCL nanofibers. (a) HCT116-19 cells (1 × 10⁶) that have undergone gene editing with 72 NT were plated onto a polylysine-coated electrospun PCL nanofiber loose membrane. Forty-eight hours after the plating, cells were observed under a Leica SP5 TCSII multiphoton confocal microscope. Cells expressing eGFP were found on nanofibers in a typical view. (b) Confocal image of the four (created) cells expressing eGFP (side view, b), showing the flatter side of cells attached to the nanofiber and the bulgy free surface of the cells. (c) Image of the same two cells on the right (side view) at a higher magnification (×40 objective). eGFP, enhanced green fluorescent protein; NT, nontranscribed; PCL, polycaprolactone.