Figure 5.

Genetically modified HCT116-19 cells plated onto polylysine-coated 6-well dishes for recovery and proliferation. (a) HCT116-19 cells (1 × 10⁶) that have undergone gene editing with 72 NT were plated onto polylysine-coated 6-well dishes. Cells were monitored between 96 and 240 hours, and imaged using a Zeiss Axiovert 25 CFL inverted light microscope (Zeiss). (b) HCT116-19 cells, treated in the same fashion as (in a) above, from an independent group of experiments were monitored and imaged between 96 and 216 hours using the same imaging strategy as in a. NT, nontranscribed.