FIG. 2.

The effect of Bcl-2 overexpression on cell growth and death under hypoxic or serum-depleted conditions. C2C12/Bcl-2 cells were cultured in hypoxic culture under 1% O₂ (A) or serum-depleted culture without fetal bovine serum (B). The cells were collected periodically, and the numbers of viable and dead cells were counted using trypan blue dye-exclusion method to calculate the percentages of cell growth (left) and death (right). Open columns, without Dox addition; closed columns, with Dox addition. The data are expressed as mean±SD (n=3). *p<0.05 versus the culture in the absence of Dox.