FIG. 1.

Morphology and surface marker profile characterization of human adipose tissue-derived stem cells (hASCs) and SSEA-4⁺hASCs. (A) Cell surface marker profile of stromal vascular fraction (SVF), obtained by flow cytometry. Optical micrographs showing the morphology of hASCs (B, C) and SSEA-4⁺hASCs (E, F) cultured in alpha-minimum essential medium (α-MEM) at Passage 0 (P0) and 4 (P4). Immunocytochemistry of selected SSEA-4⁺cells cultured for 3 days in α-MEM confirming the majority of the cells expressing SSEA-4 (D). Cell nuclei were counterstained with 4, 6-diamidino-2-phenyindole dilactate (DAPI). Scale bar represents 100 μm. Color images available online at www.liebertpub.com/tea