FIG. 7.

Angiopoietin1/2 expression by HUVEC and hES-MC. ELISA analysis for expression of (A) Ang1 and (B) Ang2 for EM control, conditioned EM from coculture of HUVEC and hES-MC, HUVEC alone, or hES-MC alone. EM registered background levels of 26±5 pg/mL and 622±101 pg/mL, while coculture (Co) measured 159±15 pg/mL and 30,867±2685 pg/mL, which were both significantly greater than EM control (*p=0.004 and 0.01). Conditioned media from HUVEC alone were not different compared with EM control for Ang1 (25±4 pg/mL) but showed significantly more expression of Ang2 than EM control (30,867±2685 pg/mL, ^#p=0.01). In contrast, conditioned media from hES-MC alone were significantly enriched in Ang1 (100±18 pg/mL, ^#p=0.02) but not in Ang2 (600±144 pg/mL). (C) HUVEC were seeded in collagen-Fn gels at 10⁶/mL and cultured in EM only (left), CM incubated with IgG-Fc (middle), or CM incubated with Tie2-Fc (right). (D) Mean network length quantification (±SEM) and one-way ANOVA indicate that exposure to hES-MC CM significantly increased network formation compared with EM control (*p>0.05), but no significant difference was detected by treatment with Tie2-Fc (494±58 μm) compared with IgG-Fc (505±59 μm) control (p=0.4) (HUVEC UEA-fluorescein, n=3; 10×, scale bar=200 μm). Color images available online at www.liebertpub.com/tea