FIG. 8.
hES-MC contact with EC networks in vivo. (A) HUVEC-DsRed and hES-MC-GFP (2×106:0.4×106/mL) were cocultured in GFR-Matrigel for 3 days in vitro before subcutaneous implantation in the back of Rag1 immune compromised mice for 1 week. Constructs were harvested and images were acquired by confocal microscopy and stack volume rendering. hES-MC integrate into EC networks that were formed in vivo (arrows). (B) Quantification of the percentage of direct cell contact with respect to HUVEC (cell overlap/HUVEC volume) and direct cell contact with respect to hES-MC (cell overlap/hES-MC volume) (40×, scale bar=50 μm). Color images available online at www.liebertpub.com/tea