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. 2004 Mar;48(3):930–939. doi: 10.1128/AAC.48.3.930-939.2004

FIG. 5.

FIG. 5.

Southern analysis of the ceaS2-Fs mutant. (A) Diagram of the ceaS2 region of the S. clavuligerus chromosome in the wild type, the ceaS2::apr mutant, and the ceaS2-Fs mutant. The gray arrow represents the apr disruption cassette, and the open arrow represents ceaS2 with the direction of transcription represented by the direction of the arrowheads. The solid bar represents the frame shift mutation, and the fine lines represent the rest of the S. clavuligerus chromosome. (B) Southern analysis of EcoRI- and NruI-digested genomic DNA from S. clavuligerus wild-type and ceaS2 mutant strains. DNA from the wild type, from ceaS2::apr mutants 4B and 4B-C, and from ceaS2-Fs mutants FS3, FS7, FS8, and FS10 was probed with a ceaS2-specific probe and an apr-specific probe.