Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Clavulanic acid production by single and double mutants of ORFs located in the paralogue gene cluster
| Wild type or mutant | Clavulanic acid produceda (% relative to wild type)
|
|
|---|---|---|
| SA medium | Soy medium | |
| Wild type | 100 | 100 |
| ceaS2-Fs | 0 | 5-13 |
| ΔceaS1::apr | 17-63 | 2-63 |
| ceaS2-Fs/ΔceaS1::apr | 0 | 0 |
| bls1::tsr | 103-162 | 107-173 |
| bls1::tsr/bls2::apr | 0 | 0 |
| oat1::neo | 30-154 | 57-145 |
| oat1::neo/oat2::apr | 12-62 | 31-67 |
The amount of clavulanic acid produced after 96 h of growth was measured by HPLC analysis.
