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. 2012 Nov 22;41(Database issue):D666–D675. doi: 10.1093/nar/gks1119

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© The Author(s) 2012. Published by Oxford University Press.

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Figure 4. — Organization of the edited SEVA plasmid replication origins. The R6K suicide origin lacks the gene encoding the Π replication protein, which makes its maintenance dependent on the trans delivery of that protein. RK2 is formed by the vegetative origin (oriV) and the replication protein trfA. pBBR1 has a similar structure to RK2; however, the pRO1600/ColE1 origin is a hybrid of the pRO1600 origin and the ColE1 replication sequence. For RSF1010, the mob genes have been eliminated and the synthetic sequence has only the oriV and the repBAC genes, which are expressed from a PlacUV5 promoter.