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. 2012 Nov 22;41(Database issue):D666–D675. doi: 10.1093/nar/gks1119

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com.

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Figure 5. — Combination of expression and reporter cargos in the SEVA plasmids. (a) The structure of the default cloning cargo with the universal M13 primers (F24 and R24) and relevant restriction sites. (b) Assembling expression systems as PacI/AvrII fragments removes the R24 primer sequence and SfiI sites upstream of the AvrII site. (c) Cloning of reporter genes as HindIII/SpeI fragments removes the F24 primer sequence and a NotI site that is placed downstream of the HindIII site. (d) Combination of an expression system and reporter gene leaves an intact polylinker (from EcoRI to HindIII) and a unique NotI site between the AvrII and EcoRI sequences, which can be used to clone large constructs.