Figure 1.

Sequences of putative dienelactone hydrolases where aligned with Clustal Omega (49). The Family Tree was built with FastTree 2 (50), and branches were coloured according to the nature of the nucleophilic residue in the active site. Two families were separated, in blue and red the Dienelactone_hydrolase family and in green the DLH-S family. Blue branches are sequences that have a Cys suitably positioned to be the nucleophilic active site residue, whereas green and red branches have a Ser. Black branches are sequences that have neither Cys nor Ser.