Table 1.
Summary of current data in NHPRTR. The 40.5 billion reads span three different library preparation methods and two sequencing instruments (GAIIx and the HiSeq2000)
| Species | File size (GB) | HiSeq2000 (2 × 100 nt paired-end reads) GAII (100 nt single-end reads) |
|||
|---|---|---|---|---|---|
| Protocol | Number of read pairs | Protocol | Number of reads | ||
| Baboon | 973 | mRNA-seq | 955 573 799 | mRNA-seq | 71 477 607 |
| UDG mRNA-seq | 918 735 897 | UDG mRNA-seq | 67 763 503 | ||
| Total RNA-seq | 151 524 634 | ||||
| Chimpanzee | 94.9 | Total RNA-seq | 198 954 000 | ||
| 399.1 | UDG mRNA-seq | 836 864 082 | |||
| Cynomolgus Macaque Indochinese | 948 | mRNA-seq | 923 307 160 | mRNA-seq | 72 016 960 |
| UDG mRNA-seq | 894 367 594 | UDG mRNA-seq | 63 820 198 | ||
| Total RNA-seq | 157 762 299 | ||||
| Cynomolgus Macaque Mauritian | 656 | mRNA-seq | 503 249 742 | mRNA-seq | 90 166 271 |
| UDG mRNA-seq | 557 450 722 | UDG mRNA-seq | 91 262 189 | ||
| Total RNA-seq | 176 108 975 | ||||
| Gorilla | 98.5 | Total RNA-seq | 206 526 535 | ||
| 422.6 | UDG mRNA-seq | 886 261 413 | |||
| Japanese Macaque | 986 | mRNA-seq | 942 269 530 | mRNA-seq | 77 740 433 |
| UDG mRNA-seq | 943 158 996 | UDG mRNA-seq | 72 925 864 | ||
| Total RNA-seq | 181 184 542 | ||||
| Marmoset | 128.8 | Total RNA-seq | 269 969 905 | ||
| 418.9 | UDG mRNA-seq | 878 369 246 | |||
| Mouse Lemur | 97.5 | Total RNA-seq | 204 494 231 | ||
| 378.9 | UDG mRNA-seq | 794 659 816 | |||
| Pig-tailed Macaque | 951 | mRNA-seq | 867 009 248 | mRNA-seq | 54 292 043 |
| UDG mRNA-seq | 991 993 458 | UDG mRNA-seq | 54 668 320 | ||
| Total RNA-seq | 131 548 564 | ||||
| Rhesus Macaque Chinese | 700 | mRNA-seq | 644 468 744 | mRNA-seq | 77 142 089 |
| UDG mRNA-seq | 661 177 666 | UDG mRNA-seq | 75 142 089 | ||
| Total RNA-seq | 121 570 595 | ||||
| Rhesus Macaque Indian | 1331.2 | mRNA-seq | 1 716 083 364 | mRNA-seq | 84 892 037 |
| UDG mRNA-seq | 704 493 397 | UDG mRNA-seq | 70 346 332 | ||
| Total RNA-seq | 168 710 995 | ||||
| Ring-tailed Lemur | 104.8 | Total RNA-seq | 219 647 886 | ||
| 398.7 | UDG mRNA-seq | 835 972 568 | |||
| Sooty Mangabey | 106 | Total RNA-seq | 222 192 769 | ||
| 424.4 | UDG mRNA-seq | 889 864 522 | |||
| Total | 9618.3 | 18 667 116 290 | 2 112 066 539 | ||
| Total number of reads | 39 446 299 119 | ||||
Although the sequencing for all species is not identical, due to increased use of the higher output HiSeq2000 in later species, we represent both total RNA and polyA-enriched RNA preparations for all species.