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. 2012 Dec 27;7(12):e52682. doi: 10.1371/journal.pone.0052682

Figure 4. CD271 expression in pancreatic stellate cells (PSCs) decreases after long-term interactions with pancreatic cancer cells.

Figure 4

(A) Real-time qRT-PCR analyses showed that the levels of CD271 mRNA expression in PSCs in coculture with pancreatic cancer cells start to increase on day 3 (p = 0.0249), are highest on day 4 (p<0.0001), and then decrease the following day after the peak expression (p<0.0001). (B) In cultures with addition of cancer cell-derived supernatant, the level of CD271 mRNA expression started to increase at day 2 (p<0.0001) and then decreased on day 4 (p<0.0001). (C) Evaluation of the differences in CD271 expression depending on the function of PSCs by real-time qRT-PCR. The level of CD271 mRNA expression in top side-derived PSCs, which did not move toward cancer cells through Matrigel and remained on upper chambers, is lower than that in bottom side-derived PSCs, which did move toward pancreatic cancer cells through Matrigel (p<0.01). *p<0.05; **p<0.01; ***p<0.001; NS, not significant.