Figure 5. Cytoskeleton Beta-tubulin changes in electroporation- or electrochemotherapy-treated HMEC-1 monolayers.

High magnification pictures (40×) of Beta-tubulin stained monolayers cultured on glass chamber slides were taken at 10 min, 2 hrs and 24 hrs post-EP with indicated E, either without (EP) or in the presence of 300 nM bleomycin (ECT). Green is fluorescein stained Beta-tubulin using a double immunostaining protocol, red is DNA/RNA stained with PI. Bar = 50 µm.