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. 2012 Dec 27;7(12):e53212. doi: 10.1371/journal.pone.0053212

Table 1. Primers used in the study a.

Primer Sequence (5′ to 3′)a
P1F ATGGTACCAGGAAGTGAAGGCAT
P1R ACGCTAGCAAGCTGGATGCAATA
P2F AACCATGGCAAGGCCAAAGCTTTGAG
P2R ACGGATCCAACAGAACTTGGAATATCATC
P3F CAGTAAGAGAACACAAGCTAATTACT
P3R GTCGCAAGTTTGCATTTATCATCT
P4F AACAGGATCCTAGGTAATACCCGAGCTTC
P4R AACCATGGTCTGACGCTCAGTGGAA
P5F TCACGGTGTTATGGAAATAG
P5R GACTGCGAGATCATAGATATAG
P6F AAGGATCCTTGGAGGAAATTGATGGAA
P6R CCTCTAGACCACTGACTTACAAGTAGAC
P7F AAGGTACCAAGATAGAGAGAGAAAAGTG
P7R TAGGATCCTTAAGGTTTTTTTGGACTTTCT
P8F AAGGATCCTAAATTTAATAGAAGGAGGAA
P8R AACTGCAGGTCTTGATTATCGGGCGAAGAG
a

The underlined sequences are restriction enzyme sites: Acc65I sites (P1F and P7F), BamHI sites (P2R, P4F, P6F, P7R and P8F), NcoI sites (P2F and P4R), a NheI site (P1R), a PstI site (P8R), and an XbaI site (P6R).