Table 1. Primers used in the study a.
Primer | Sequence (5′ to 3′)a |
P1F | ATGGTACCAGGAAGTGAAGGCAT |
P1R | ACGCTAGCAAGCTGGATGCAATA |
P2F | AACCATGGCAAGGCCAAAGCTTTGAG |
P2R | ACGGATCCAACAGAACTTGGAATATCATC |
P3F | CAGTAAGAGAACACAAGCTAATTACT |
P3R | GTCGCAAGTTTGCATTTATCATCT |
P4F | AACAGGATCCTAGGTAATACCCGAGCTTC |
P4R | AACCATGGTCTGACGCTCAGTGGAA |
P5F | TCACGGTGTTATGGAAATAG |
P5R | GACTGCGAGATCATAGATATAG |
P6F | AAGGATCCTTGGAGGAAATTGATGGAA |
P6R | CCTCTAGACCACTGACTTACAAGTAGAC |
P7F | AAGGTACCAAGATAGAGAGAGAAAAGTG |
P7R | TAGGATCCTTAAGGTTTTTTTGGACTTTCT |
P8F | AAGGATCCTAAATTTAATAGAAGGAGGAA |
P8R | AACTGCAGGTCTTGATTATCGGGCGAAGAG |
The underlined sequences are restriction enzyme sites: Acc65I sites (P1F and P7F), BamHI sites (P2R, P4F, P6F, P7R and P8F), NcoI sites (P2F and P4R), a NheI site (P1R), a PstI site (P8R), and an XbaI site (P6R).