Table 2. Constructs and clones used in the study.
| Construct or clone | Description | Source |
| pBBE22 | pBSV2 carrying a bbe22 copy | Reference [27] |
| pME22a | pBSV2 carrying a bbe22 copy | Reference [19] |
| pBBE22-ospC′ | pBBE22 carrying promoterless ospC fused with flaB promoter | Reference [28] |
| pME22-C′B′A′ | pME22 carrying promoterless ospC, dbpB and dbpA fused with flaB promoter | This study |
| 13A | Cloned from the B. burgdorferi B31 5A13 | Reference [25] |
| ΔrpoS | rpoS mutant | This study |
| ΔrpoS/rpoS | ΔrpoS receiving pBBE22 carrying a wild-type rpoS copy | This study |
| ΔrpoS/E22 | ΔrpoS receiving pBBE22 | This study |
| ΔrpoS/ospC′ | ΔrpoS expressing ospC driven by flaB promoter | This study |
| ΔrpoS/C′B′A′ | ΔrpoS expressing ospC, dbpB and dbpA driven by flaB promoter | This study |
a
pME22 was modified from pBBE22 by removing the bbe22 copy from the Acc65I site to the AatII in order to make the Acc65I site available for cloning [19].