Figure 2. Supernatant fluids early post infection contain infectious virus.

All in all, 15 BM samples from healthy rhesus monkeys were studied as described in Figure 1. (A) Kinetics of viral replication in 15 bone marrow cell cultures. Red line indicates RNA titers and green line indicates NS1 protein levels. (B) Infectious virus recovery from supernatant fluids. Supernatants from days 2 (red line) and 5 (green line) were cultured with Vero cells. Approximately equal amounts of viral RNA from 5 randomly chosen monkey BM supernatant fluids were utilized in these culture experiments. Supernatant from day 2 contained infectious dengue virus.