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. 2012 Dec 27;7(12):e52902. doi: 10.1371/journal.pone.0052902

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© 2012 Clark et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Bone marrow smears were prepared and fluorescent cell stainings were performed as described in the Methods. (A) Dengue viral E antigen (identified by 4G2) in tetraploid megakaryocyte in the process of shedding vesicles as evidenced by immunohistochemical staining in the presence of DAPI. Dengue viral antigen (red) and nucleus (blue) (B) Dengue NS1 antigen in a CD61+ megakaryocytic cell depicted by immunofluorescence staining. NS1 (green), CD61 (red) and nucleus (blue). Scale bar,10 µm.