Table 2. Infectivity of dengue virus in Colony Forming Unit cells picked from human bone marrowa.
Days P.I. | 0b | 1 | 3 | 5 | 7 | 10 |
CFU-other cells | 74c | 215 | 198 | 136 | 124 | 103 |
Fold Increased | 0 | 190.5 | 167.6 | 83.3 | 67.6 | 39.2 |
CFU-Megakaryocytes | 82 | 114 | 363 | 145 | 92 | 26 |
Fold Increase | 0 | 39.5 | 344.3 | 77.5 | 12.1 | 0 |
CFU-Erythroid | 3530 | 1000 | 657 | 433 | 194 | 0 |
Fold Increase | 0 | 0 | 0 | 0 | 0 | 0 |
Cells were characterized based-upon their morphology and giemsa staining characteristics.
Day 0 means the time point 2 hours after adsorption, in which culture supernatants were extensively washed for unbound virus. The amount of residual virus in the culture supernatant was determined and used as the baseline.
Quantification determined by qRT-PCR (unadjusted copy number per 140 µlof the supernatant).
The fold increase relative to the viral titer at Day 0, supernatant at 2 hours post-infection, was calculated.